Imaging and mapping the impact of clouds on skyglow with all-sky photometry

Artificial skyglow is constantly growing on a global scale, with potential ecological consequences ranging up to affecting biodiversity. To understand these consequences, worldwide mapping of skyglow for all weather conditions is urgently required. In particular, the amplification of skyglow by clouds needs to be studied, as clouds can extend the reach of skyglow into remote areas not affected by light pollution on clear nights. Here we use commercial digital single lens reflex cameras with fisheye lenses for all-sky photometry. We track the reach of skyglow from a peri-urban into a remote area on a clear and a partly cloudy night by performing transects from the Spanish town of Balaguer towards Montsec Astronomical Park. From one single all-sky image, we extract zenith luminance, horizontal and scalar illuminance. While zenith luminance reaches near-natural levels at 5km distance from the town on the clear night, similar levels are only reached at 27km on the partly cloudy night. Our results show the dramatic increase of the reach of skyglow even for moderate cloud coverage at this site. The powerful and easy-to-use method promises to be widely applicable for studies of ecological light pollution on a global scale also by non-specialists in photometry.Comment: 13 pages, 7 figure

Light pollution offshore: Zenithal sky glow measurements in the mediterranean coastal waters

Light pollution is a worldwide phenomenon whose consequences for the natural environment and the human health are being intensively studied nowadays. Most published studies address issues related to light pollution inland. Coastal waters, however, are spaces of high environmental interest, due to their biodiversity richness and their economical significance. The elevated population density in coastal regions is accompanied by correspondingly large emissions of artificial light at night, whose role as an environmental stressor is increasingly being recognized. Characterizing the light pollution levels in coastal waters is a necessary step for protecting these areas. At the same time, the marine surface environment provides a stage free from obstacles for measuring the dependence of the skyglow on the distance to the light polluting sources, and validating (or rejecting) atmospheric light propagation models. In this work we present a proof-of-concept of a gimbal measurement system that can be used for zenithal skyglow measurements on board both small boats and large vessels under actual navigation conditions. We report the results obtained in the summer of 2016 along two measurement routes in the Mediterranean waters offshore Barcelona, travelling 9 and 31.7¿km away from the coast. The atmospheric conditions in both routes were different from the ones assumed for the calculation of recently published models of the anthropogenic sky brightness. They were closer in the first route, whose results approach better the theoretical predictions. The results obtained in the second route, conducted under a clearer atmosphere, showed systematic differences that can be traced back to two expected phenomena, which are a consequence of the smaller aerosol content: the reduction of the anthropogenic sky glow at short distances from the sources, and the slower decay rate of brightness with distance, which gives rise to a relative excess of brightness at large distances from the coastlinePeer ReviewedPostprint (author's final draft

Effects of parasite and historic driven selection on the diversity and structure of a MHC-II gene in a small mammal species (Peromyscus leucopus) undergoing range expansion.

Abstract Genetic diversity may decrease from the centre to the margin of a species distribution range due to neutral stochastic processes. Selection may also alter genetic diversity in non-neutral markers, such as genes associated with the immune system. Both neutral processes and selection on the immune system are thus expected to affect the spatial distribution of such markers, but the relative strength of each has been scarcely studied. Here, we compared the diversity of a neutral marker (mitochondrial cytochrome b)and a selected marker (DRB gene from the MHC-II), in eastern-North American populations of white-footed mice (Peromyscus leucopus), a species known for its role of main reservoir of the Lyme disease. We observed distinct phylogeographic patterns with these two markers, which may be the result of selection pressure acting upon the DRB gene. As predicted by the central marginal hypothesis, we observed a loss of neutral genetic diversity toward the margin of the species distribution. A decrease in diversity was also observed for the DRB gene, likely due to genetic drift and positive selection operated by helminth parasites. Such a loss in genetic diversity at the range margin may slow down the ongoing expansion of P. leucopus, by counterbalancing the effect of global warming on the mouse survival at higher latitude

Modelling the night sky brightness and light pollutionsources of Montsec protected area

We proceeded to the modelling of the night sky brightness of Montsec area (north-east of Spain), an astronomical protected area certified as a Starlight Reserve. We have used the hyperspectral version of ILLUMINA, an artificial sky brightness model. Ground based measurements for Montsec and other areas of Catalonia [15], [16], including both photometric and spectroscopic data, has been used to fit and evaluate the input parameters of the model. In this first modelling attempt, Lleida, the biggest city in the area, has been considered as the unique source of light pollution. In 2014 there was an update of the lighting infrastructure in Lleida. A detailed comparison of the sky brightness before and after the change is shown in order to measure the effects that different kind of lamps can produce. This information could be used to plan for future updates and improvements of the lighting systems in the area.Peer ReviewedPostprint (author's final draft

Testing parasite 'intimacy': the whipworm Trichuris muris in the European house mouse hybrid zone.

Host-parasite interaction studies across hybrid zones often focus on host genetic variation, treating parasites as homogeneous. 'Intimately' associated hosts and parasites might be expected to show similar patterns of genetic structure. In the literature, factors such as no intermediate host and no free-living stage have been proposed as 'intimacy' factors likely constraining parasites to closely follow the evolutionary history of their hosts. To test whether the whipworm, Trichuris muris, is intimately associated with its house mouse host, we studied its population genetics across the European house mouse hybrid zone (HMHZ) which has a strong central barrier to gene flow between mouse taxa. T. muris has a direct life cycle and nonmobile free stage: if these traits constrain the parasite to an intimate association with its host we expect a geographic break in the parasite genetic structure across the HMHZ. We genotyped 205 worms from 56 localities across the HMHZ and additionally T. muris collected from sympatric woodmice (Apodemus spp.) and allopatric murine species, using mt-COX1, ITS1-5.8S-ITS2 rDNA and 10 microsatellites. We show four haplogroups of mt-COX1 and three clear ITS1-5.8S-ITS2 clades in the HMHZ suggesting a complex demographic/phylogeographic history. Microsatellites show strong structure between groups of localities. However, no marker type shows a break across the HMHZ. Whipworms from Apodemus in the HMHZ cluster, and share mitochondrial haplotypes, with those from house mice. We conclude Trichuris should not be regarded as an 'intimate' parasite of the house mouse: while its life history might suggest intimacy, passage through alternate hosts is sufficiently common to erase signal of genetic structure associated with any particular host taxon

Tracking the dynamics of skyglow with differential photometry using a digital camera with fisheye lens

Artificial skyglow is dynamic due to changing atmospheric conditions and the switching on and off of artificial lights throughout the night. Street lights as well as the ornamental illumination of historical sites and buildings are sometimes switched off at a certain time to save energy. Ornamental lights in particular are often directed upwards, and can therefore have a major contribution towards brightening of the night sky. Here we use differential photometry to investigate the change in night sky brightness and illuminance during an automated regular switch-off of ornamental light in the town of Balaguer and an organized switch-off of all public lights in the village of \`Ager, both near Montsec Astronomical Park in Spain. The sites were observed during two nights with clear and cloudy conditions using a DSLR camera and a fisheye lens. A time series of images makes it possible to track changes in lighting conditions and sky brightness simultaneously. During the clear night, the ornamental lights in Balaguer contribute over 20% of the skyglow at zenith at the observational site. Furthermore, we are able to track very small changes in the ground illuminance on a cloudy night near \`Ager.Comment: 17 pages, 13 figures, 1 tabl

Helminth communities of the exotic introduced turtle, Trachemys scripta elegans in southwestern Spain: Transmission from native turtles

We report the prevalence and diversity of helminth parasites found in native turtles Mauremys leprosa and Emys orbicularis from three localities in southwestern Spain and we describe the helminth communities of exotic turtles Trachemys scripta elegans coexisting in the wild with both native turtle species. Five nematodes species were identified, of which Serpinema microcephalus was the only species common between two localities, although infection parameters were different between them. This is the first report of cross transmission of S. microcephalus and Falcaustra donanaensis from native to exotic turtles and the first report of genus Physaloptera in turtles of the Palearctic Region. Continuous releasing of exotic pet turtles in wildlife ecosystems increases the risk of parasite introductions and, consequently, potential transmission to native species, and highlights the impending need for regulation of pet turtle trade in Europe

Abnormalities in gray matter volume in patients with borderline personality disorder and their relation to lifetime depression: A VBM study

Background Structural imaging studies of borderline personality disorder (BPD) have found regions of reduced cortical volume, but these have varied considerably across studies. Reduced hippocampus and amygdala volume have also been a regular finding in studies using conventional volumetric measurement. How far comorbid major depression, which is common in BPD and can also affect in brain structure, influences the findings is not clear. Methods Seventy-six women with BPD and 76 matched controls were examined using whole-brain voxel-based morphometry (VBM). The hippocampus and amygdala were also measured, using both conventional volume measurement and VBM within a mask restricted to these two subcortical structures. Lifetime history of major depression was assessed using structured psychiatric interview. Results At a threshold of p = 0.05 corrected, the BPD patients showed clusters of volume reduction in the dorsolateral prefrontal cortex bilaterally and in the pregenual/subgenual medial frontal cortex. There was no evidence of volume reductions in the hippocampus or amygdala, either on conventional volumetry or using VBM masked to these regions. Instead there was evidence of right-sided enlargement of these structures. No significant structural differences were found between patients with and without lifetime major depression. Conclusions According to this study, BPD is characterized by a restricted pattern of cortical volume reduction involving the dorsolateral frontal cortex and the medial frontal cortex, both areas of potential relevance for the clinical features of the disorder. Previous findings concerning reduced hippocampus and amygdala volume in the disorder are not supported. Brain structural findings in BPD do not appear to be explainable on the basis of history of associated lifetime major depression

Determination of neuronal antibodies in suspected and definite Creutzfeldt-Jakob disease

IMPORTANCE Creutzfeldt-Jakob disease (CJD) and autoimmune encephalitis with antibodies against neuronal surface antigens (NSA-abs) may present with similar clinical features. Establishing the correct diagnosis has practical implications in the management of care for these patients. OBJECTIVE To determine the frequency of NSA-abs in the cerebrospinal fluid of patients with suspected CJD and in patients with pathologically confirmed (ie, definite) CJD. DESIGN, SETTING, AND PARTICIPANTS A mixed prospective (suspected) and retrospective (definite) CJD cohort study was conducted in a reference center for detection of NSA-abs. The population included 346 patients with suspected CJD and 49 patients with definite CJD. MAIN OUTCOMES AND MEASURES Analysis of NSA-abs in cerebrospinal fluid with brain immunohistochemistry optimized for cell-surface antigens was performed. Positive cases in the suspected CJD group were further studied for antigen specificity using cell-based assays. All definite CJD cases were comprehensively tested for NSA-abs, with cell-based assays used for leucine-rich glioma-inactivated 1 (LGI1), contactin-associated protein-like 2 (CASPR2), N-methyl-D-aspartate (NMDA), and glycine (GlY) receptors. RESULTS Neuronal surface antigens were detected in 6 of 346 patients (1.7%) with rapid neurologic deterioration suggestive of CJD. None of these 6 patients fulfilled the diagnostic criteria for probable or possible CJD. The target antigens included CASPR2, LGI1, NMDAR, aquaporin 4, Tr (DNER [δ/notch-like epidermal growth factor-related receptor]), and an unknown protein. Four of the patients developed rapidly progressive dementia, and the other 2 patients had cerebellar ataxia or seizures that were initially considered to be myoclonus without cognitive decline. The patient with Tr-abs had a positive 14-3-3 test result. Small cell lung carcinoma was diagnosed in the patient with antibodies against an unknown antigen. All patients improved or stabilized after appropriate treatment. None of the 49 patients with definite CJD had NSA-abs. CONCLUSIONS AND RELEVANCE A low, but clinically relevant, number of patients with suspected CJD had potentially treatable disorders associated with NSA-abs. In contrast, none of 49 patients with definite CJD had NSA-abs, including NMDAR-abs, GlyR-abs, LGI1-abs, or CASPR2-abs. These findings suggest that cerebrospinal fluid NSA-abs analysis should be included in the diagnostic workup of patients with rapidly progressive central nervous system syndromes, particularly when they do not fulfill the diagnostic criteria of probable or possible CJD